Anti-LYN (Phospho-Y397) Antibody

SKU: orb315590

Description

Rabbit polyclonal antibody to LYN (Phospho-Y397) also known as p53Lyn and p56Lyn whose expression can be detected in the placenta and fetal brain but is highly expressive in the neuroblastoma tumors and neuronal cells. It is found in the perinuclear region. This protein is a Non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors and plays an important role in the regulation of innate and adaptive immune responses, hematopoiesis, responses to growth factors and cytokines, integrin signaling, but also responses to DNA damage and genotoxic agents.

Research Area

Infectious Diseases,Immunology,Product Categories/Research Area,Product Categories/Antibodies,Epigenetics,Product Categories/Products/Antibodies/Primary Antibodies,Product Categories/Products/Antibodies,Cancer

Images & Validation

• Tested Applications: IF, IH, WB
• Dilution Range: WB: 1-500:1000, IHC-P: 1-100:200, IF/ICC: 1-100:500
• Reactivity: Human, Mouse, Porcine, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y397 of human LYN protein. The exact sequence is proprietary.
• Target: LYN
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti-JTK8 antibody, anti-Tyrosine-protein kinase Lyn antibody, anti-Lck/Yes-related novel protein tyrosine kinase antibody, anti-V-yes-1 Yamaguchi sarcoma viral related oncogene homolog antibody, anti-p53Lyn antibody, anti-p56Lyn antibody

Western blot analysis of LYN (Phospho-Y397) expression in Jurkat (A), human spleen (B), mouse lung (C) whole cell lysates. (Predicted band size: 58 kD; Observed band size: 58 kD)

Immunohistochemical analysis of LYN (Phospho-Y397) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of LYN (Phospho-Y397) staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Direct ELISA antibody dose-response curve using Anti-LYN (Phospho-Y397) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.

Quick Database Links

Gene Symbol

LYN

UniProt

P07948, Q07014, P25911

NCBI Gene ID

81515, 4067, 17096