Anti-MARK Antibody

SKU: orb737145

Description

Rabbit polyclonal antibody to MARK1

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Neuroscience,Signaling Pathways,Product Categories/Products/Antibodies/Primary Antibodies,Signaling Pathways/Wnt,Epigenetics

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: WB: 1/500 - 1/1000, IF: 1/50 - 1/200
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Clone No.: MARK1; MARK2; MARK3; MARK4
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-MARK1 antibody, anti-KIAA1477 antibody, anti-MARK antibody, anti-Serine/threonine-protein kinase MARK1 antibody, anti-MAP/microtubule affinity-regulating kinase 1 antibody, anti-PAR1 homolog c antibody, anti-Par-1c antibody, anti-Par1c antibody, anti-MARK2 antibody, anti-EMK1 antibody, anti-Serine/threonine-protein kinase MARK2 antibody, anti-ELKL motif kinase 1 antibody, anti-EMK-1 antibody, anti-MAP/microtubule affinity-regulating kinase 2 antibody, anti-PAR1 homolog antibody, anti-PAR1 homolog b antibody, anti-Par-1b antibody, anti-Par1b antibody, anti-MARK3 antibody, anti-CTAK1 antibody, anti-EMK2 antibody, anti-MAP/microtubule affinity-regulating kinase 3 antibody, anti-C-TAK1 antibody, anti-cTAK1 antibody, anti-Cdc25C-associated protein kinase 1 antibody, anti-ELKL motif kinase 2 antibody, anti-EMK-2 antibody, anti-Protein kinase STK10 antibody, anti-Ser/Thr protein kinase PAR-1 antibody, anti-Par-1a antibody, anti-Serine/threonine-protein kinase p78 antibody, anti-MARK4 antibody, anti-KIAA1860 antibody, anti-MARKL1 antibody, anti-MAP/microtubule affinity-regulating kinase 4 antibody, anti-MAP/microtubule affinity-regulating kinase-like 1 antibody

Western blot analysis of MARK expression in Panc1 (A), HEK293T (B), mouse brain (C), rat brain (D) whole cell lysates.

Immunohistochemical analysis of MARK staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of MARK staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

UniProt

Q9P0L2