Anti-MARK3 Antibody

SKU: orb256677

Description

Rabbit polyclonal antibody to MARK3

Research Area

Product Categories/Research Area,Product Categories/Antibodies,Epigenetics,Product Categories/Products/Antibodies/Primary Antibodies,Product Categories/Products/Antibodies

Images & Validation

• Tested Applications: IF, IH, WB
• Dilution Range: WB: 1:500-1000, IHC-P: 1:100-200, IF/ICC: 1:100-500
• Reactivity: Human, Mouse, Rat, Zebrafish

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human MARK3. The exact sequence is proprietary.
• Target: MARK3
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti CTAK1 antibody, anti EMK2 antibody, anti MAP/microtubule affinity-regulating kinase 3 antibody, anti C-TAK1 antibody, anti cTAK1 antibody, anti Cdc25C-associated protein kinase 1 antibody, anti ELKL motif kinase 2 antibody, anti EMK-2 antibody, anti Protein kinase STK10 antibody, anti Ser/Thr protein kinase PAR-1 antibody, anti Par-1a antibody, anti Serine/threonine-protein kinase p78 antibody

Western blot analysis of MARK3 expression in SHSY5Y (A), H9C2 (B), BV2 (C) whole cell lysates. (Predicted band size: 84 kD; Observed band size: 90 kD)

Immunohistochemical analysis of MARK3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of MARK3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

MARK3

UniProt

P27448, Q03141, Q8VHF0

NCBI Gene ID

17169, 170577, 4140

Sun, Xuehua et al. Exogenous TIPE2 Inhibit TAK1 to Improve Inflammation and Neuropathic Pain Induced by Sciatic Nerve Injury Through Inactivating NF-?B and JNK Neurochem Res, (2022)

PubMed: 35842555