Anti-mCherry purified

SKU: orb763362

Description

Rabbit polyclonal antibody to mCherry

Images & Validation

• Tested Applications: FC, ICC, WB
• Dilution Range: FC: 1-4 μg/ml, WB: 1-2 μg/ml
• Application Notes:
  Flow cytometry: Recommended dilution: 1-4 μg/ml, extracellular staining or intracellular staining - depending on expression. Western blotting: Recommended dilution: 1-2 μg/ml.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Rabbit polyclonal
• Clone No.: PAb (918)
• Immunogen: mCherry protein from Anaplasma marginale
• Target: mCherry
• Purity: Purified by ligand affinity chromatography.
• Purification: Purified by ligand affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Flow cytometry surface staining pattern of HEK293T/17 cells co-transfected with mCherry/GPI and YFP/GPI constructs stained using anti-mCherry Purified rabbit polyclonal antibody (concentration in sample 2 μg/ml, GAR APC).

Separation of HEK293T/17 cells co-transfected with mCherry/GPI and YFP/GPI constructs stained anti-mCherry Purified rabbit polyclonal antibody (concentration in sample 2 μg/ml, GAR APC, red-filled) from HEK293T/17 cells co-transfected with mCherry/GPI and YFP/GPI constructs unstained by primary polyclonal antibody (GAR APC, black-dashed) in flow cytometry analysis (surface staining) of HEK293T17/mCherry/YFP cell suspension.

Western blotting analysis of mCherry fluorescent protein using rabbit polyclonal PAb (918) on lysates of HEK293T/17 cells co-transfected with mCherry/GPI and YFP/GPI constructs (HEK293T/17 cells transfected with YFP/GPI; negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of rabbit anti-mCherry polyclonal antibody followed by IRDye800-conjugated anti-rabbit secondary antibody. A specific band was detected for mCherry protein at approximately 30 kDa.

Quick Database Links

Gene Symbol

mCherry

UniProt

X5DSL3