Anti-mTOR (Phospho-S2448) Antibody

SKU: orb315804

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Description

Rabbit polyclonal antibody to MTOR and is also known as LST8 and MLST8. Diseases associated with MLST8 include Benign Familial Neonatal Epilepsy and Childhood Absence Epilepsy. Among its related pathways are Constitutive Signaling by AKT1 E17K in Cancer and RET signaling. Q9BVC4 Subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at Thr-389, which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, LST8 interacts directly with MTOR and enhances its kinase activity. In nutrient-poor conditions, it stabilizes the MTOR-RPTOR interaction and favors RPTOR-mediated inhibition of MTOR activity. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 Ser-473 phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on Thr-308 by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at Ser-422. mTORC2 also modulates the phosphorylation of PRKCA on Ser-657.

Research Area

Product Categories/Research Area,Product Categories/Antibodies,Epigenetics,Product Categories/Products/Antibodies/Primary Antibodies,Product Categories/Products/Antibodies

Images & Validation

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Tested Applications	IF, IH, WB
Dilution Range	WB: 1-500:1000, IHC-P: 1-100:200, IF/ICC: 1-100:500, IP: 1-10:100
Reactivity	Human, Mouse, Rat, Sheep

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Immunogen	KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S2448 of human mTOR protein. The exact sequence is proprietary.
Target	MTOR
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti-FRAP antibody, anti-FRAP1 antibody, anti-FRAP2 antibody, anti-RAFT1 antibody, anti-RAPT1 antibody, anti-Serine/threonine-protein kinase mTOR antibody, anti-FK506-binding protein 12-rapamycin complex-associated protein 1 antibody, anti-FKBP12-rapamycin complex-associated protein antibody, anti-Mammalian target of rapamycin antibody, anti-mTOR antibody, anti-Mechanistic target of rapamycin antibody, anti-Rapamycin and FKBP12 target 1 antibody, anti-Rapamycin target protein 1 antibody

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