Anti-Nectin-4(enfortumab biosimilar) mAb

SKU: orb1291227

Description

Anti-Nectin-4(enfortumab biosimilar) mAb

Research Area

Product Categories/Products/Antibodies,Biosimilar reference antibodies

Images & Validation

• Tested Applications: ELISA, FC
• Dilution Range: ELISA 1:5000-10000|Flow Cyt 1:100
• Reactivity: Human
• Application Notes:
  Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.

Key Properties

• Antibody Type: Biosimilar Antibody
• Host: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Target: NECTIN4
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Powder
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

EDSS1;LNIR;nectin-4;PRR4;PVRL4

ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human NECTIN-4 Protein, His Tag orb1173741 can bind Anti-Nectin-4 Neutralizing antibody (orb1291227) in a linear range of 0.61–39.06 ng/ml.

Flow cytometry analysis with 1 μg/mL Anti-Nectin-4 (enfortumab biosimilar) mAb (orb1291227) on Expi293 cells transfected with Human Nectin-4 protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

Flow cytometry analysis of antigen binding of anti-human Nectin-4 mAb (orb1291227). (A) orb1291227 does not bind to 293T cells that do not express Nectin-4. (B) A clear peak shift of orb1291227 was seen compared to the control when incubated with Nectin-4-expressing A431 cells, indicating strong binding of orb1291227 to Nectin-4. Antibodies were incubated at 2 μg/mL.

Quick Database Links

Gene Symbol

NECTIN4

UniProt

Q96NY8