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Description
Images & Validation
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Item 1 of 2
| Tested Applications | IHC, WB |
|---|---|
| Dilution Range | Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rat Immunohistochemistry (Frozen Section), 1-2μg/ml, Human, mouse, rat, -Western blot, 0.5ml, Human, mouse, rat |
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | N52 |
| Immunogen | C-terminal segment of enzymatically dephosphorylated pig Neurofilament 200. |
| Molecular Weight | 200 kDa |
| Purification | Ascites |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Concentration | Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Neurofilament heavy polypeptide; NF-H; 200 kDa neurofilament protein; Neurofilament triplet H protein; Nefh; Nfh
Quality Guarantee
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Quick Database Links
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
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Lingyi Kong 1, Heidi Olze 1, Agnieszka J Szczepek 2 3 Histamine H3 Receptor Antagonists Influence the Directional Growth of Type II Spiral Ganglion Neurites Within the Developing Cochlea of C57BL/6 Mice Neurochem Res, (2025)
Applications
IF
Reactivity
Mouse
Zhao, Quanlai et al. Cytoprotective effects of myristicin against hypoxia‑induced apoptosis and endoplasmic reticulum stress in rat dorsal root ganglion neurons Mol Med Rep, 15, 2280-2288 (2017)
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Currently estimated at 1-3 months