Anti-NET1 Antibody

SKU: orb411918

Description

Rabbit polyclonal antibody to NET1

Research Area

Product Categories/Research Area,Product Categories/Antibodies,Epigenetics,Product Categories/Products/Antibodies/Primary Antibodies,Product Categories/Products/Antibodies,Cancer

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB: 1:500-2000, IF/ICC: 1:50-200
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant full length protein of human NET1
• Target: NET1
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti-ARHGEF8 antibody, anti-Neuroepithelial cell-transforming gene 1 protein antibody, anti-Proto-oncogene p65 Net1 antibody, anti-Rho guanine nucleotide exchange factor 8 antibody

Western blot analysis of NET1 expression in Jurkat (A), HEK293T (B) whole cell lysates. (Predicted band size: 67 kD; Observed band size: 67 kD)

Immunofluorescent analysis of NET1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Quick Database Links

Gene Symbol

NET1

UniProt

Q7Z628, Q9Z206

NCBI Gene ID

56349, 10276