Anti-p53 Biotin

SKU: orb2653067

Description

The tumour suppressor protein p53 is a key element of intracellular anticancer protection. It mediates cell cycle arrest or apoptosis in response to DNA damage or to starvation for pyrimidine nukleotides. It is up-regulated in response to these stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest, or proteins that trigger apoptosis, such as Bcl-2. The structure of p53 comprises N-terminal transactivation domain, central DNA-binding domain, oligomerisation domain, and C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization.

Images & Validation

• Tested Applications: ELISA, FC, ICC, IHC-P, IP, WB
• Reactivity: Human, Primate
• Application Notes:
  Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining.

Key Properties

• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Clone No.: BP53-12
• Immunogen: Bacterially expressed full-length wild-type p53
• Target: p53
• Conjugation: Biotin

Storage & Handling

• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BCC7, TRP53, TP53, LFS1

Quick Database Links

Gene Symbol

p53

UniProt

P04637

NCBI Gene ID

7157