Anti-PAK1/2/3 Antibody

SKU: orb737136

Description

Rabbit polyclonal antibody to PAK1

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Cancer,Neuroscience,Signaling Pathways,Product Categories/Products/Antibodies/Primary Antibodies,Signaling Pathways/Apoptotic,Epigenetics

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: WB: 1/500 - 1/1000, IF: 1/50 - 1/200
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Clone No.: PAK1; PAK2; PAK3
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-PAK1 antibody, anti-Serine/threonine-protein kinase PAK 1 antibody, anti-Alpha-PAK antibody, anti-p21-activated kinase 1 antibody, anti-PAK-1 antibody, anti-p65-PAK antibody, anti-PAK2 antibody, anti-Serine/threonine-protein kinase PAK 2 antibody, anti-Gamma-PAK antibody, anti-PAK65 antibody, anti-S6/H4 kinase antibody, anti-p21-activated kinase 2 antibody, anti-PAK-2 antibody, anti-p58 antibody, anti-PAK3 antibody, anti-OPHN3 antibody, anti-Serine/threonine-protein kinase PAK 3 antibody, anti-Beta-PAK antibody, anti-Oligophrenin-3 antibody, anti-p21-activated kinase 3 antibody, anti-PAK-3 antibody

Western blot analysis of PAK1/2/3 expression in A375 (A), A549 (B) whole cell lysates.

Immunohistochemical analysis of PAK1/2/3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of PAK1/2/3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

UniProt

Q13153