Anti-PRG2 Antibody

SKU: orb340847

Description

Rabbit polyclonal antibody to PRG2

Research Area

Immunology,Product Categories/Research Area,Product Categories/Antibodies,Epigenetics,Product Categories/Products/Antibodies/Primary Antibodies,Product Categories/Products/Antibodies

Images & Validation

• Tested Applications: IF, IH, WB
• Dilution Range: WB: 1:500-1:2000, IHC-P: 1:50-1:200
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant full length protein of human PRG2
• Target: PRG2
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MBP; Bone marrow proteoglycan; BMPG; Proteoglycan 2

Western blot analysis of PRG2 expression in HEK293T (A), H1792 (B), mouse spleen (C), mouse liver (D), rat spleen (E), rat liver (F) whole cell lysates. (Predicted band size: 25; 23 kD; Observed band size: 33; 31 kD)

Immunohistochemical analysis of PRG2 staining in human gastric cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of PRG2 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

PRG2

UniProt

P13727, Q61878

NCBI Gene ID

19074, 5553