Anti-Pyruvate Kinase Antibody

SKU: orb1474211

Description

Mouse monoclonal antibody to Pyruvate Kinase

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100)
• Reactivity: Human, Mouse, Primate, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: Purified recombinant human PKM2 protein fragments expressed in E.coli.
• Target: PKM
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

OIP3; PK2; PK3; PKM2; Pyruvate kinase PKM; Cytosolic thyroid hormone-binding protein; CTHBP; Opa-interacting protein 3; OIP-3; Pyruvate kinase 2/3; Pyruvate kinase muscle isozyme; Thyroid hormone-binding protein 1; THBP1; Tumor M2-PK; p58

Western blot analysis of Pyruvate Kinase expression in COS7 (A), PC12 (B), C6 (C), NIH3T3 (D), Hela (E) whole cell lysates. (Predicted band size: 58 kD; Observed band size: 58 kD)

Immunofluorescent analysis of Pyruvate Kinase staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

Quick Database Links

Gene Symbol

PKM

UniProt

P14618, P52480, P11980

NCBI Gene ID

25630, 18746, 5315