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Description
Research Area
Product Categories/Products/Antibodies,Product Categories/Products/Antibodies/Primary Antibodies
Images & Validation
−
| Tested Applications | ELISA, IP, WB |
|---|---|
| Dilution Range | WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature). IS: 1:500-2000 dilution. For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test. |
| Reactivity | Other |
| Application Notes |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | RF5R |
| Immunogen | RFP from the Discosoma sp. (sea anemone) N-terminal peptide-KLH conjugates. |
| Purity | Protein A affinity chromatography from mouse ascites fluid. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS, pH 7.4 with 0.05% sodium azide. |
| Concentration | 100ug/100ul |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IP
Immunoprecipitation
Anti-RFP Tag (Mouse Monoclonal) (orb1140403)
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