Anti-RUNX2 Antibody

SKU: orb213669

Description

Rabbit polyclonal antibody to RUNX2

Research Area

Product Categories/Research Area,Product Categories/Antibodies,Epigenetics,Product Categories/Products/Antibodies/Primary Antibodies,Product Categories/Products/Antibodies

Images & Validation

• Tested Applications: IF, IH, WB
• Dilution Range: WB: 1-500-1-1000, IHC-P: 1-100-1-200
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human RUNX2. The exact sequence is proprietary.
• Target: RUNX2
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti Acute myeloid leukemia 3 protein antibody, anti alpha subunit 1 antibody, anti AML3 antibody, anti CBF alpha 1 antibody, anti CBFA1 antibody, anti CCD antibody, anti CCD1 antibody, anti cleidocranial dysplasia 1 antibody, anti Core binding factor antibody, anti Core binding factor runt domain alpha subunit 1 antibody, anti Core binding factor subunit alpha 1 antibody, anti Oncogene AML 3 antibody, anti OSF 2 antibody, anti OSF-2 antibody, anti OSF2 antibody, anti Osteoblast specific transcription factor 2 antibody, anti PEA2 alpha A antibody, anti PEA2aA antibody, anti PEBP2 alpha A antibody, anti PEBP2-alpha A antibody, anti PEBP2A1 antibody, anti PEBP2A2 antibody, anti Polyomavirus enhancer binding protein 2 alpha A subunit antibody, anti runt domain antibody, anti Runt related transcription factor 2 antibody, anti RUNX2 antibody, anti SL3 3 enhancer factor 1 alpha A subunit antibody, anti SL3/AKV core binding factor alpha A subunit antibody

Western blot analysis of RUNX2 expression in HEK293T (A), DLD (B), mouse spleen (C) whole cell lysates. (Predicted band size: 56 kD; Observed band size: 57 kD)

Immunohistochemical analysis of RUNX2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of RUNX2 staining in B12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

RUNX2

UniProt

Q08775, Q9Z2J9, Q13950

NCBI Gene ID

860, 12393