Anti-SUMO2/3 Antibody

SKU: orb214592

Description

Rabbit polyclonal antibody to SUMO2

Research Area

Product Categories/Research Area,Product Categories/Antibodies,Epigenetics,Product Categories/Products/Antibodies/Primary Antibodies,Product Categories/Products/Antibodies

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB: 1-500-1-1000
• Reactivity: Human, Mouse, Porcine, Rat, Zebrafish

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SUMO2/3. The exact sequence is proprietary.
• Target: SUMO2
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti SMT3A antibody, anti SMT3H2 antibody, anti Small ubiquitin-related modifier 2 antibody, anti SUMO-2 antibody, anti HSMT3 antibody, anti SMT3 homolog 2 antibody, anti SUMO-3 antibody, anti Sentrin-2 antibody, anti Ubiquitin-like protein SMT3A antibody, anti Smt3A antibody

Western blot analysis of SUMO2/3 expression in HEK293T (A), A549 (B), U2OS (C), mouse brain (D), mouse testis (E), rat brain (F), rat testis (G) whole cell lysates. (Predicted band size: 10; 11 kD; Observed band size: 17 kD)

Immunofluorescent analysis of SUMO2/3 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

SUMO2

UniProt

P61959, P61956, P61957

NCBI Gene ID

170930, 6613, 690244