Anti-TBX18 Antibody

SKU: orb214859

Description

Rabbit polyclonal antibody to TBX18

Research Area

Product Categories/Research Area,Product Categories/Antibodies,Product Categories/Products/Antibodies/Primary Antibodies,Developmental Biology,Product Categories/Products/Antibodies

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB: 1-500-1-1000
• Reactivity: Human, Mouse, Rat, Zebrafish

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human TBX18. The exact sequence is proprietary.
• Target: TBX18
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti T-box transcription factor TBX18 antibody, anti T-box protein 18 antibody

Western blot analysis of TBX18 expression in U2OS (A), mouse muscle (B), mouse heart (C), rat muscle (D) whole cell lysates. (Predicted band size: 64 kD; Observed band size: 65 kD)

Immunofluorescent analysis of TBX18 staining in A2780 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

TBX18

UniProt

Q9EPZ6, O95935

NCBI Gene ID

9096, 76365