Anti-TRAP220 Antibody

SKU: orb214424

Description

Rabbit polyclonal antibody to MED1

Research Area

Product Categories/Research Area,Product Categories/Antibodies,Epigenetics,Product Categories/Products/Antibodies/Primary Antibodies,Developmental Biology,Product Categories/Products/Antibodies

Images & Validation

• Tested Applications: IF, IH, WB
• Dilution Range: WB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500
• Reactivity: Human, Mouse, Primate, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human TRAP220. The exact sequence is proprietary.
• Target: MED1
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti ARC205 antibody, anti CRSP1 antibody, anti CRSP200 antibody, anti DRIP205 antibody, anti DRIP230 antibody, anti PBP antibody, anti PPARBP antibody, anti PPARGBP antibody, anti RB18A antibody, anti TRAP220 antibody, anti TRIP2 antibody, anti Mediator of RNA polymerase II transcription subunit 1 antibody, anti Activator-recruited cofactor 205 kDa component antibody, anti ARC205 antibody, anti Mediator complex subunit 1 antibody, anti Peroxisome proliferator-activated receptor-binding protein antibody, anti PBP antibody, anti PPAR-binding protein antibody, anti Thyroid hormone receptor-associated protein complex 220 kDa component antibody, anti Trap220 antibody, anti Thyroid receptor-interacting protein 2 antibody, anti TR-interacting protein 2 antibody, anti TRIP-2 antibody, anti Vitamin D receptor-interacting protein complex component DRIP205 antibody

Western blot analysis of TRAP220 expression in H446 (A) whole cell lysates. (Predicted band size: 168 kD; Observed band size: 220 kD)

Immunohistochemical analysis of TRAP220 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of TRAP220 staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Quick Database Links

Gene Symbol

MED1

UniProt

Q15648, Q925J9

NCBI Gene ID

5469, 19014