Anti-TWIST Antibody

SKU: orb214705

Description

Rabbit polyclonal antibody to TWIST1

Research Area

Product Categories/Research Area,Product Categories/Antibodies,Product Categories/Products/Antibodies/Primary Antibodies,Developmental Biology,Product Categories/Products/Antibodies

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB: 1-500-1-1000
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human TWIST. The exact sequence is proprietary.
• Target: TWIST1
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti BHLHA38 antibody, anti TWIST antibody, anti Twist-related protein 1 antibody, anti Class A basic helix-loop-helix protein 38 antibody, anti bHLHa38 antibody, anti H-twist antibody

Western blot analysis of TWIST expression in Jurkat (A), Raw264.7 (B), rat liver (C) whole cell lysates. (Predicted band size: 20 kD; Observed band size: 23 kD)

Immunofluorescent analysis of TWIST staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

TWIST1

UniProt

P26687, Q15672

NCBI Gene ID

22160, 7291