Anti-UDG Antibody

SKU: orb214719

Description

Rabbit polyclonal antibody to UNG

Research Area

Infectious Diseases,Product Categories/Research Area,Product Categories/Antibodies,Cancer/DNA Damage,Epigenetics,Product Categories/Products/Antibodies/Primary Antibodies,Product Categories/Products/Antibodies,Cancer

Images & Validation

• Tested Applications: IF, IH, WB
• Dilution Range: WB: 1-500-1-1000, IHC-P: 1-100-1-200
• Reactivity: Human, Mouse, Porcine, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human UDG. The exact sequence is proprietary.
• Target: UNG
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti DGU antibody, anti UNG1 antibody, anti UNG15 antibody, anti Uracil-DNA glycosylase antibody, anti UDG antibody

Western blot analysis of UDG expression in PC2 (A), SGC7901 (B), HEK293T (C), PC12 (D), CT26 (E) whole cell lysates. (Predicted band size: 33; 34 kD; Observed band size: 35 kD)

Immunohistochemical analysis of UDG staining in human muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of UDG staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

UNG

UniProt

P13051, P97931

NCBI Gene ID

22256, 7374