Anti-VEGFR2 (Phospho-Y1175) Antibody

SKU: orb256644

Description

Rabbit polyclonal antibody to VEGFR2 (phospho-Y1175)

Research Area

Infectious Diseases,Product Categories/Research Area,Product Categories/Antibodies,Epigenetics,Product Categories/Products/Antibodies/Primary Antibodies,Developmental Biology,Product Categories/Products/Antibodies,Cancer

Images & Validation

• Tested Applications: IF, IH, WB
• Dilution Range: WB: 1:500-1000, IHC-P: 1:100-200, IF/ICC: 1:100-500
• Reactivity: Human, Mouse, Porcine, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y1175 of human VEGFR2 protein. The exact sequence is proprietary.
• Target: KDR
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti CD309 antibody, anti CD309 antigen antibody, anti Fetal liver kinase 1 antibody, anti FLK-1 antibody, anti FLK1 antibody, anti FLK1, mouse, homolog of antibody, anti KDR antibody, anti Kinase insert domain receptor (a type III receptor tyrosine kinase) antibody, anti Kinase insert domain receptor antibody, anti KRD1 antibody, anti Ly73 antibody, anti Protein tyrosine kinase receptor FLK1 antibody, anti Protein-tyrosine kinase receptor flk-1 antibody, anti soluble VEGFR2 antibody, anti Tyrosine kinase growth factor receptor antibody, anti Vascular endothelial growth factor receptor 2 antibody, anti VEGFR 2 antibody, anti VEGFR antibody, anti VEGFR-2 antibody, anti VEGFR2 antibody

Western blot analysis of VEGFR2 (Phospho-Y1175) expression in HEK293T EGF-treated (A), NIH3T3 EGF-treated (B) whole cell lysates. (Predicted band size: 151 kD; Observed band size: 230 kD)

Immunohistochemical analysis of VEGFR2 (Phospho-Y1175) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of VEGFR2 (Phospho-Y1175) staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

KDR

UniProt

O08775, P35968, P35918

NCBI Gene ID

25589, 3791

McClellan, Eric B. et al. l-Citrulline treatment alters the structure of the pulmonary circulation in hypoxic newborn pigs Pediatr Pulmonol, 55, 2762-2772 (2020)

PubMed: 32662946