Anti-ZAP70 Antibody

SKU: orb705545

Description

Mouse monoclonal antibody to ZAP70

Research Area

Immunology,Product Categories/Research Area,Product Categories/Antibodies,Epigenetics,Product Categories/Products/Antibodies/Primary Antibodies,Product Categories/Products/Antibodies

Images & Validation

• Tested Applications: IH, WB
• Dilution Range: WB: 1:500-1:1000, IHC-P: 1:100-1:300
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human ZAP70. The exact sequence is proprietary.
• Target: ZAP70
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Mouse IgG2b. Liquid in PBS containing 50% glycerol, 0.2% rAlbumin and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-SRK antibody, anti-Tyrosine-protein kinase ZAP-70 antibody, anti-70 kDa zeta-chain associated protein antibody, anti-Syk-related tyrosine kinase antibody

Western blot analysis of ZAP70 expression in JurKat (A) whole cell lysates. (Predicted band size: 69 kD; Observed band size: 69 kD)

Immunohistochemical analysis of ZAP70 staining in human hodgkin's lymphoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Quick Database Links

Gene Symbol

ZAP70

UniProt

P43403

NCBI Gene ID

7535