AP2A1/AP2-Alpha Antibody

SKU: orb1539391

Description

AP2A1/AP2-Alpha Antibody

Research Area

Product Categories/Products/Antibodies

Images & Validation

• Tested Applications: ELISA, ICC, IHC, IHC-P, WB
• Dilution Range: ICC, IHC, IHC-P (5 µg/ml), Peptide-ELISA (1:128000), WB (0.01 - 0.03 µg/ml)
• Reactivity: Canine, Equine, Human, Monkey, Mouse, Porcine, Rabbit
• Application Notes:
  Further information: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 110kD band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 108kD according to NP_055018.2). The same band is observed in Mouse Brain, but not in Mouse Liver or Mouse Kidney lysates. Recommended concentration: 0.01-0.03 ug/ml. Immunocytochemistry: HeLa stably expressing a chimeric construct including the brain-specific splice variant of Mouse Ap2a1, showing clathrin-coated pits and vesicles budding from the plasma membrane.

  Read more...

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-ELEPPAPESPMALLADPAPAAD, from the internal region of the protein sequence according to NP_055018.2.
• Target: AP2A1 / AP2-Alpha
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Concentration: 0.5 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AP2A1, Adaptin, alpha A, ADTAA, Alpha-adaptin A, Alpha1-adaptin, AP-2 complex subunit alpha-1, AP2 Alpha, CLAPA1, AP2-alpha

Quick Database Links

Gene Symbol

AP2A1 / AP2-Alpha