APOBEC3G Antibody

SKU: orb1246654

Description

APOBEC3G Antibody

Research Area

,Infectious Disease

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx. 45kDa band observed in lysates of cell line Daudi (calculated MW of 46.4kDa according to NP_068594.1). Recommended concentration: 0.5-1.5ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Testis and Kidney. Recommended concentration: 2.5ug/ml.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-DEHSQDLSGRLR
• Target: APOBEC3G
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

APOBEC3G, apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G, ARP9, CEM15, FLJ12740, MDS019, bK150C2.7, dJ494G10.1 , DNA dC->dU editing enzyme, OTTHUMP00000028911, apolipoprotein B mRNA editing enzyme catalytic polypeptide-like 3G, phorbolin-like protein MDS019

orb1246654 (0.5 ug/ml) staining of Daudi lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246654 (2.5 ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1246654 (2.5 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

APOBEC3G

UniProt

Q9HC16

RefSeq (Protein):NP_068594.1