ATF6 Antibody (Center)

SKU: orb1928352

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

,Primary Antibodies;Catalog Updated;Apoptosis signaling;Unfolded protein response

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: WB: 1:1000, WB: 1:16000, IHC-P: 1:10~50, FC: 1:10~50
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB22035
• Target: This ATF6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 350-377 amino acids of human ATF6.
• Molecular Weight: 74585 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cyclic AMP-dependent transcription factor ATF-6 alpha, cAMP-dependent transcription factor ATF-6 alpha, Activating transcription factor 6 alpha, ATF6-alpha, Processed cyclic AMP-dependent transcription factor ATF-6 alpha, ATF6

Western blot analysis of ATF6 Antibody (Center) in A2058 cell line lysates (35 ug/lane). ATF6 (arrow) was detected using the purified Pab.

ATF6 Antibody (Center) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Anti-ATF6 Antibody (Center) at 1: 16000 dilution + Hela whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 75 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human brain tissue reacted with ATF6 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Quick Database Links

Gene Symbol

This ATF6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 350-377 amino acids of human ATF6.

UniProt

P18850

RefSeq (Protein):NP_031374.2