ATG5 Antibody (C-term)

SKU: orb33367

Description

Purified rabbit polyclonal antibody.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Cancer,Cardiovascular Disease,Neuroscience,Signaling Pathways,Signaling Pathways/Apoptotic,Signaling Pathways/Autophagic,Immunology,Epigenetics,Infectious Diseases,Crown Antibodies;Primary Antibodies;Cancer;Cardiovascular;Cell Biology;Metabolism;Immunity;Innate immunity;RIG-l-like Receptor;Autophagy;Regulation of autophagy

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: IF/ICC: 1:25, WB: 1:1000, IHC-P: 1:50-100
• Reactivity: Human, Mouse

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB7547
• Immunogen: Synthetic Peptide
• Target: ATG5
• Molecular Weight: 32447
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-APG5 antibody, anti-APG5-LIKE antibody, anti-APG5L antibody, anti-ASP antibody, anti-hAPG5 antibody, anti-APG5 (autophagy 5 antibody, anti-S. cerevisiae)-like antibody, anti-APG5 autophagy 5-like antibody, anti-ATG5 autophagy related 5-like antibody, anti-OTTHUMP00000017824 antibody, anti-apoptosis specific protein antibody, anti-apoptosis-specific protein antibody, anti-autophagy protein 5 antibody

Cos7, HEK293, MEF, and Hela cells left to right, respectively.

Western blot analysis of APG5L Pab in Y79 cell line, mouse liver tissue, and Hela cell line lysates.

Western blot analysis of APG5L (arrow) using rabbit polyclonal APG5L Antibody (P224). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APG5L gene.

Fluorescent image of U251 cells stained with ATG5 Antibody (C-term). Diluted at 1:25 dilution. U251 cells were treated with Chloroquine (50 μM, 16 h), An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Quick Database Links

Gene Symbol

ATG5

UniProt

Q9H1Y0

RefSeq (Protein):NP_004840.1