ATG5 Antibody (N-term)

SKU: orb33366

Description

Purified rabbit polyclonal antibody.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Cancer,Cardiovascular Disease,Neuroscience,Signaling Pathways,Signaling Pathways/Apoptotic,Signaling Pathways/Autophagic,Immunology,Epigenetics,Infectious Diseases,Primary Antibodies;Antibody Collections;Cancer;Cardiovascular;Cell Biology;Metabolism;Autophagy;Immunity;Innate immunity;RIG-l-like Receptor;Autophagy;Regulation of autophagy

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: WB: 1:1000, IF/ICC: 1:200, IHC-P: 1:50-100
• Reactivity: Human, Mouse
• Predicted Reactivity: Porcine, Rat, Zebrafish

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB7466
• Immunogen: Synthetic Peptide
• Target: ATG5
• Molecular Weight: 32447
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-APG5 antibody, anti-APG5-LIKE antibody, anti-APG5L antibody, anti-ASP antibody, anti-hAPG5 antibody, anti-APG5 (autophagy 5 antibody, anti-S. cerevisiae)-like antibody, anti-APG5 autophagy 5-like antibody, anti-ATG5 autophagy related 5-like antibody, anti-OTTHUMP00000017824 antibody, anti-apoptosis specific protein antibody, anti-apoptosis-specific protein antibody, anti-autophagy protein 5 antibody

The anti-APG5L Pab is used in Western blot to detect APG5L in mouse brain tissue lysate.

All lanes: Anti-hAPG5L-D3 at 1:1000 dilution. Lane 1: SH-SY5Y whole cell lysate. Lane 2: Hela whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 32 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded human liver tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Fluorescent image of U251 cells stained with ATG5 (N-term) antibody. U251 cells were treated with Chloroquine (50 μM, 16 h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with ATG5 (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1 h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). ATG5 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

Quick Database Links

Gene Symbol

ATG5

UniProt

Q9H1Y0

RefSeq (Protein):NP_004840.1