ATG5 (Autophagy Marker) Antibody

SKU: orb2316102

Description

ATG5 (Autophagy Marker) Antibody

Research Area

,Autophagy|Immunology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA (For coating use Ab at 1-5ug/ml, order Ab without BSA); Western Blot (1-2ug/ml);Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
• Reactivity: Human
• Application Notes:
  Positive Control: THP-1, PANC-1, Raji or HeLa cells. Brain, Ovary, Endometrium, Colon or Duodenum (IHC).

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: ATG5/2101
• Immunogen: Recombinant fragment of human ATG5 protein (around aa 1-119) (exact sequence is proprietary)
• Target: ATG5
• Source/Expression System: Bioreactor
• Molecular Weight: 50-32kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Concentration: Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Autophagy protein 5 (APG5); APG5 autophagy 5 like (APG5L); Apoptosis-specific protein (ASP); ATG5; Autophagy related 5; hAPG5

Quick Database Links

Gene Symbol

ATG5

UniProt

Q9H1Y0

NCBI Gene ID

9474