ATG7 Antibody (C-term)

SKU: orb1433690

Description

Rabbit polyclonal antibody to ATG7.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Cancer,Neuroscience,Signaling Pathways,Signaling Pathways/Autophagic,Epigenetics,Primary Antibodies;Antibody Collections;Cancer;Cardiovascular;Cell Biology;Metabolism;Signal Transduction;Autophagy;Autophagy;Regulation of autophagy

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: IF: 1:25, WB: 1:1000, WB: 1:1000, WB: 1:500, IHC-P-Leica: 1:1000
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB7474
• Immunogen: Synthetic Peptide
• Target: ATG7
• Molecular Weight: 77960
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Ubiquitin-like modifier-activating enzyme ATG7, ATG12-activating enzyme E1 ATG7, Autophagy-related protein 7, APG7-like, hAGP7, Ubiquitin-activating enzyme E1-like protein, ATG7, APG7L

All lanes: Anti-ATG7 Antibody (C-term) at 1:1000 dilution. Lane 1: HepG2 whole cell lysate. Lane 2: NIH/3T3 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 78 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-ATG7 Antibody (C-term) at 1:500 dilution. Lane 1: Caki-1 whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: HepG2 whole cell lysate.Lysates/proteins at 40 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 78 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunofluorescent analysis of U251 cells, using ATG7 Antibody (C-term). U251 cells (right) were treated with Chloroquine (50 μM, 16h). Diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).

Immunohistochemical analysis of paraffin-embedded human liver tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Western blot analysis of APG7L Pab in 293 cell line lysate (35 ug/lane). APG7L (arrow) was detected using the purified Pab.

Quick Database Links

Gene Symbol

ATG7

UniProt

O95352

RefSeq (Protein):NP_001129503.2, NP_006386.1, NP_001138384.1