ATG7 Antibody: HRP

SKU: orb55135

Description

Rabbit polyclonal antibody against ATG7 conjugated to HRP.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Cancer,Neuroscience,Signaling Pathways,Product Categories/Products/Antibodies/Primary Antibodies,Signaling Pathways/Autophagic,Epigenetics,Cancer | Apoptosis | Autophagy | Cardiovascular System | Heart

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: WB (1:1000); ICC/IF (1:100); IHC (1:50)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  A 1:1000 dilution of SPC-610 was sufficient for detection of ATG7 in 15 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from the N-terminal of Human ATG7
• Target: ATG7
• Molecular Weight: 77.9 kDa
• Conjugation: HRP

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide and 0.23mM Citrate in dH2O
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Autophagy 7S. cerevisiae, homolog of antibody, APG7 like antibody, ATG7_HUMAN antibody, hAGP7 antibody, Ubiquitin-like modifier-activating enzyme ATG7 antibody, Autophagy related protein 7 antibody, Autophagy-related protein 7 antibody, GSA 7 antibody, APG7, S. cerevisiae, homolog of antibody, Ubiquitin-activating enzyme E1-like protein antibody, Autophagy-related 7 (yeast) antibody, APG7 autophagy 7 like antibody, 1810013K23Rik antibody, APG7 autophagy 7-like (S. cerevisiae) antibody, ATG12-activating enzyme E1 ATG7 antibody, ATG7 autophagy related 7 homolog antibody, Atg7l antibody, Ubiquitin activating enzyme E1 like protein antibody, Apg 7 antibody, APG7L antibody, DKFZp434N0735 antibody, ATG 7 antibody, APG7-like antibody, GSA7 antibody, ATG7 autophagy related 7 homolog (S. cerevisiae) antibody, ATG7 antibody

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG7 Polyclonal Antibody. Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG7 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Preautophagosomal Structure, Organelle membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ATG7 Antibody. (D) Composite.

Immunohistochemistry analysis using Rabbit Anti-ATG10 Polyclonal Antibody. Tissue: Brain. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-ATG10 Polyclonal Antibody at 1:50 for 31 min at RT. Counterstain: Hematoxylin. Magnification: 10X.

Western blot analysis of Rat brain cell lysates showing detection of ~77.9 kDa ATG7 protein using Rabbit Anti-ATG7 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat brain cell lysates. Load: 20 μg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG7 Polyclonal Antibody at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~77.9 kDa.

Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~77.9 kDa ATG7 protein using Rabbit Anti-ATG7 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Human Cervical cancer cell line (HeLa) lysate. Load: 15 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG7 Polyclonal Antibody at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~77.9 kDa.

Quick Database Links

Gene Symbol

ATG7

UniProt

O95352

NCBI Gene ID

10533

RefSeq (Protein):NP_001129503.2