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Description
Research Area
,Neuroscience,Signal Transduction
Images & Validation
−Item 1 of 8
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 230-529 of human ATP5B (NP_001677.2). |
| Target | ATP5B |
| Molecular Weight | Observed: 57kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−ATP5B, ATPMB, ATPSB, MGC5231, HEL-S-271
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Western blot analysis of extracts of various cell lines, using ATP5B Antibody (orb1257339) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Immunohistochemistry of paraffin-embedded rat heart using ATP5B antibody (orb1257339) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using ATP5B antibody (orb1257339) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using ATP5B antibody (orb1257339) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using ATP5B antibody (orb1257339) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using ATP5B antibody (orb1257339) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using ATP5B antibody (orb1257339) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using ATP5B antibody (orb1257339) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
ATP5B
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ATP5B Antibody (orb1257339)
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