B7H3 Antibody [10G6]

SKU: orb1239231

Description

B7H3 Antibody [10G6]

Research Area

,Immunology

Images & Validation

• Tested Applications: ELISA, FC, ICC, IF, IHC-P
• Reactivity: Human
• Application Notes:
  B7-H3 antibody can be used for detection of B7-H3 for immunohistochemistry starting at 2 μg/mL and Immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL. Flow cytometry at 1 μg/ml.Antibody validated: Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in human samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone No.: 10G6
• Immunogen: B7-H3 antibody was raised against the extracellular domain of human B7-H3
• Target: CD276
• Purification: B7-H3 Antibody is supplied as protein A purified IgG1.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: B7-H3 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

B7-H3 Antibody: CD276 molecule, B7H3, B7RP-2, 4Ig-B7-H3, CD276

Immunocytochemistry of B7-H3 in HEK293 cells using B7-H3 antibody and control mouse IgG antibody (left corner box) at 1 μg/ml.

Immunofluorescence of B7-H3 in HEK293 cells using B7-H3 Antibody at 5 μg/ml. Green: B7-H3 Antibody [10G6] (orb1239231) Blue: DAPI staining

Immunofluorescence of B7-H3 in human colon carcinoma tissue cells using B7-H3 Antibody at 10 μg/ml. Green: B7-H3 Antibody [10G6] (orb1239231) Blue: DAPI staining

Immunohistochemistry of B7-H3 in human colon carcinoma tissue using B7-H3 Antibody and control mouse IgG (corner box) at 2 μg/ml.

Flow cytometry analysis of B7-H3 in HEK293 cells using B7-H3 antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: B7-H3 over expressing HEK293 cells.

Titration curve analysis of B7-H3 mAbs to detect recombinant B7-H3 in ELISA with orb1240155, orb1239243, orb1239244, orb1239247, and orb1239231 antibodies at decreasing concentrations.

Quick Database Links

Gene Symbol

CD276

UniProt

Q5ZPR3

RefSeq (Protein):NP_001019907