Bax Antibody (BH3 Domain Specific)

SKU: orb31066

Description

Rabbit polyclonal antibody to BAX

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Cancer,Neuroscience,Signaling Pathways,Product Categories/Products/Antibodies/Primary Antibodies,Signaling Pathways/Apoptotic,Epigenetics,Infectious Diseases,Crown Antibodies;Primary Antibodies;Antibody Collections;Cancer;Cell Biology;Metabolism;Amyotrophic Lateral Sclerosis (ALS);Viral process;Host-virus interaction;Apoptosis;Apoptosis signaling;Mitochondrion

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: WB: 1:2000, IHC-P: 1:50-100, FACS: 1:10-50
• Reactivity: Human, Mouse

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB0952
• Immunogen: Synthetic Peptide
• Target: BAX
• Molecular Weight: 21184
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti-Apoptosis regulator BAX antibody, anti- BAX antibody, anti- BAX-protein antibody, anti- BAX_HUMAN antibody, anti- BAXA antibody, anti- BAXdelta2G9 antibody, anti- BAXdelta2G9omega antibody, anti- BAXdelta2omega antibody, anti- omega antibody, anti- transcript variant delta2 antibody, anti- membrane isoform alpha antibody

All lanes: Anti-Bax Antibody (BH3 Domain Specific) at 1:2000 dilution. Lane 1: HT-1080 whole cell lysate. Lane 2: Raji whole cell lysate. Lane 3: SH-SY5Y whole cell lysate. Lane 4: THP-1 whole cell lysate. Lane 5: NIH/3T3 whole cell lysate. Lane 6: L929 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 21 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-Bax Antibody (BH3 Domain Specific) at 1:2000 dilution. Lane 1: Raji whole cell lysate. Lane 2: SH-SH5Y whole cell lysate. Lane 3: HT-1080 whole cell lysate. Lane 4: NIH/3T3 whole cell lysate. Lane 5: L929 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 21 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-Bax Antibody (BH3) at 1:2000 dilution. Lane 1: HT-1080 whole cell lysate. Lane 2: L929 whole cell lysate. Lane 3: Raji whole cell lysate. Lane 4: SH-SY5Y whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 21 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-Bax Antibody (BH3) at 1:2000 dilution + mouse spleen lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 21 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Overlay histogram showing HeLa cells (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

Quick Database Links

Gene Symbol

BAX

UniProt

Q07812

RefSeq (Protein):NP_004315.1, NP_620119.2, NP_620118.1, NP_620116.1