BCA Protein Assay Kit

SKU: orb219872

Description

Bicinchoninic Acid Protein Assay Kit

Research Area

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Images & Validation

• Application Notes:
  Protein quantification is often required before precessing protein samples for analysis. The Bicinchoninic Acid (BCA) Protein Assay is highly sensitive colorimetric assay that is not affected by chemicals in the sample. The BCA Protein Assay primarily reduces Cu2+to Cu1+by proteins in an alkaline environment followed by highly sensitive and selective colorimetric detection of BCA/copper complex. It is water-soluble and strongly absorbs light at 562nm in a linear fashion with increasing protein concentration.

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Key Properties

• Reactivity: All
• Sample Types: All
• Range: 15.6 μg/ml - 2000 μg/ml

Storage & Handling

• Storage: Shipped and store at 4°C for 6 months.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Haiyan Xiang, Yan Wu, Yun Zhang, Yuanhao Hong & Yaling Xu Obtusifolin inhibits podocyte apoptosis by inactivating NF-κB signaling in acute kidney injury Cytotechnology, (2024)

PubMed:

Applications

WB

Reactivity

Mouse

Wei Ji # 1, Guoqing Gong # 2, Yuanhang Liu 1, Yan Liu 1, Jie Zhang 1, Qiang Li Icariin promotes osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) by activating PI3K-AKT-UTX/EZH2 signaling in steroid-induced femoral head osteonecrosis J Orthop Surg Res, (2025)

PubMed: 40098175