Bcl-2 Antibody [100/D5]

SKU: orb1252825

Description

Bcl-2 Antibody [100/D5]

Research Area

,Apoptosis,Autophagy,Cancer

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, IP, WB
• Reactivity: Human
• Application Notes:
  ELISA: order BSA-free format for coatingFlow Cytometry: 0.5-1 ug/million cellsIF: 1-2 ug/mlWB: 0.5-1 ug/mlIP: 1-2ug/0.5mg lysateIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Bcl-2 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 1mM EDTA Buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

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Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: 100/D5
• Immunogen: Amino acids GAAPAPGIFSSQPG (41-54) of the human protein were used as the immunogen for this Bcl-2 antibody.
• Target: BCL2
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Bcl-2 Antibody: Bcl-2, PPP1R50, Apoptosis regulator Bcl-2

Quick Database Links

Gene Symbol

BCL2

UniProt

P10415