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Bcl-2 (Apoptosis & Follicular Lymphoma Marker) Antibody
SKU: orb388614
Featured
Description
Research Area
Product Categories/Products/Antibodies/Primary Antibodies,Neuroscience,Signaling Pathways,Cancer,Product Categories/Antibodies,Product Categories/Products/Antibodies,Epigenetics,Signaling Pathways/Apoptotic,AKT Signaling|Apoptosis|Autophagy|Basal Cell Marker|Cardiovascular|Cytokine Signaling|Immunology|Lung Cancer|Mitochondria Marker|Neuroinflammation|Signal Transduction
Images & Validation
−| Tested Applications | FC, IF, IHC, WB |
|---|---|
| Dilution Range | FACS: 0.5-1 μg/million cells, IF/ICC: 0.5-1 μg/ml, WB: 0.5-1 μg/ml, IHC-P: 0.5-1 μg/ml |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | SPM530 |
| Immunogen | A synthetic peptide, aa41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein |
| Target | BCL2 |
| Source/Expression System | HEK293 cells |
| Molecular Weight | 25-26kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
| Buffer/Preservatives | 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
| Concentration | Purified Ab with BSA and Azide at 200ug/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Apoptosis regulator Bcl-2, B-cell CLL/lymphoma-2
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
IF
Immunofluorescence

















