BCL10 Mouse Monoclonal Antibody

SKU: orb783402

Description

BCL10 Mouse Monoclonal Antibody

Research Area

Apoptosis, Cell Cycle, Bcl-2 Family, Bcl-2 Family, Cancer Research, Metabolism, NF-kB Signalling Pathway

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1:50-100
• Reactivity: Human, Mouse
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,Κ
• Clone No.: G56
• Immunogen: KLH conjugated synthetic peptide derived from human BCL10 (1-130/233aa)
• Target: BCL10
• Molecular Weight: 26 kDa
• Purification: affinity purified by Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Bcl-10; B cell CLL/lymphoma 10; B cell lymphoma/leukemia10; B-cell leukemia/lymphoma 10; Bcl 10; c E10; c-E10; C81403; CARD containing apoptotic signaling protein; CARD containing molecule enhancing NF kappa B; CARD containing molecule enhancing NF kB; CARD containing molecule enhancing NFkB; CARD containing proapoptotic protein; CARD like apoptotic protein; CARMEN; Caspase recruiting domain containing protein; cCARMEN; cE 10; cE10; CED 3/ICH 1 prodomain homologous E10 like regulator; CED3/ICH1 prodomain homologous E10 like regulator; Cellular E10; Cellular homolog of vCARMEN; CIPER; CLAP; hCLAP; Mammalian CARD containing adapter molecule E10; mE 10; mE10; R-RCD1; BCL10_HUMAN.

Blank control: Hela. Primary Antibody (green line): Mouse Anti-BCL10 antibody (orb783402), dilution: 1:100, Secondary Antibody: Goat anti-mouse IgG-FITC, dilution: 0.5 ug/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control: Hela. Primary Antibody (green line): Mouse Anti-BCL10 antibody (orb783402), dilution: 1:50, Isotype Control Antibody (blue line): Mouse IgG, Secondary Antibody: Goat anti-mouse IgG-AF488, dilution: Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (BCL10) Monoclonal Antibody, Unconjugated (orb783402) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (BCL10) Monoclonal Antibody, Unconjugated (orb783402) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: Lane 1: Daudi cell lysates, Primary: Anti-BCL10 (orb783402) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 26 kD, Observed band size: 30 kD.

Quick Database Links

Gene Symbol

BCL10

UniProt

O95999