BCL2L1 monoclonal antibody (M01), clone 3E2-2A5

SKU: orb2295560

Description

Mouse monoclonal antibody raised against a full length recombinant BCL2L1.

Images & Validation

• Tested Applications: ELISA, IF, PLA, WB
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1 Kappa
• Clone No.: 3E2-2A5
• Immunogen: BCL2L1 (AAH19307, 1 a.a. ~ 233 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Protein Sequence: MSQSNRELVVDFLSYKLSQKGYSWSQFSDVEENRTEAPEGTESEMETPSAINGNPSWHLADSPAVNGATGHSSSLDAREVIPMAAVKQALREAGDEFELRYRRAFSDLTSQLHITPGTAYQSFEQVVNELFRDGVNWGRIVAFFSFGGALCVESVDKEMQVLVSRIAAWMATYLNDHLEPWIQENGGWDTFVELYGNNAAAESRKGQERFNRWFLTGMTVAGVVLLGSLFSRK
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

BCL2L1 monoclonal antibody (M01), clone 3E2-2A5 Western Blot analysis of BCL2L1 expression in A-431.

Detection limit for recombinant GST tagged BCL2L1 is approximately 0.03 ng/ml as a capture antibody.

Immunofluorescence of monoclonal antibody to BCL2L1 on A-431 cell. [antibody concentration 10 ug/ml].

Proximity Ligation Analysis of protein-protein interactions between BID and BCL2L1. HeLa cells were stained with anti-BID rabbit purified polyclonal 1:1200 and anti-BCL2L1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Western Blot analysis of BCL2L1 expression in transfected 293T cell line by BCL2L1 monoclonal antibody (M01), clone 3E2-2A5. Lane 1: BCL2L1 transfected lysate (Predicted MW: 26 KDa). Lane 2: Non-transfected lysate.

Western Blot detection against Immunogen (51.37 KDa).

Quick Database Links

RefSeq:AAH19307