Beta-actin Antibody (Ascites)

SKU: orb2649354

Description

Mouse Monoclonal Antibody (Mab)

Research Area

,Primary Antibodies;Antibody Collections;Cancer;Signal Transduction;Loading Control

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB: 1:1000, WB: 1:5000~20000, IHC-P: 1:10~50, IHC-P: 1:10~50
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: 8H10D10
• Target: ACTB recombinant protein is used to produce this monoclonal antibody.
• Molecular Weight: 41737 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Actin, cytoplasmic 1, Beta-actin, Actin, cytoplasmic 1, N-terminally processed, ACTB

All lanes: Anti-Beta-actin Antibody at 1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: NIH/3T3 whole cell lysate. Lane 3: HepG2 whole cell lysate. Lane 4: MCF-7 whole cell lysate. Lane 5: A431 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/8000 dilution.Observed band size: 42 KDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human heart tissue reacted with Beta-actin Monoclonal Antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Beta-actin Monoclonal Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of anti-Beta-actin Monoclonal Antibody in HL-60 cell line lysates (35 μg/lane). Beta-actin (arrow) was detected using the purified Mab.

Quick Database Links

Gene Symbol

ACTB recombinant protein is used to produce this monoclonal antibody.

UniProt

P60709

RefSeq (Protein):NP_001092.1