beta-Actin Rabbit pAb, Loading Control Antibody

SKU: orb2563021

Description

beta-Actin Rabbit pAb, Loading Control Antibody

Images & Validation

• Tested Applications: ICC, IF, WB
• Dilution Range: WB=1:500-5000, IF=1:10-20, ICC/IF=1:50
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Feline, Gallus, Hamster, Mouse, Porcine, Rabbit, Rat, Sheep, Zebrafish

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Synthetic MAP peptide derived from human beta-Actin (1-200/375aa)
• Molecular Weight: 42 kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Beta Actin; beta-Actin; ACTB; Actin cytoplasmic 1; Actin, beta; Beta actin; beta cytoskeletal actin; A X actin like protein; ACTB; Actin cytoplasmic 1; alpha sarcomeric Actin; Actx; Beta cytoskeletal actin; Melanoma X actin; PS1TP5BP1; ACTB_HUMAN.

25 ug total protein per lane of various lysates probed with beta-Actin polyclonal antibody, unconjugated (orb2563021) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Quick Database Links

UniProt

P60709