BID Antibody

SKU: orb349098

Description

Mouse monoclonal antibody to BID.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Cancer,Neuroscience,Signaling Pathways,Product Categories/Products/Antibodies/Primary Antibodies,Signaling Pathways/Apoptotic,Epigenetics,Crown Antibodies;Primary Antibodies;Cancer;Cell Biology;Metabolism;Apoptosis;Mitochondrion

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: IF/ICC: 1:25, IHC-P: 1:25, WB: 1:4000
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone No.: 1519CT649.18.47
• Target: BID
• Molecular Weight: 21995
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti Apoptic death agonist antibody, anti BH3 interacting domain death agonist antibody, anti BH3 interacting domain death agonist p11 antibody, anti BH3 interacting domain death agonist p13 antibody, anti BH3 interacting domain death agonist p15 antibody, anti BH3-interacting domain death agonist p11 antibody, anti BID antibody, anti BID isoform ES(1b) antibody, anti BID isoform L(2) antibody, anti BID isoform Si6 antibody, anti Desmocollin type 4 antibody, anti FP497 antibody, anti p11 BID antibody, anti p13 BID antibody, anti p15 BID antibody, anti p22 BID antibody

All lanes: Anti-BID Antibody at 1:4000 dilution. Lane 1: Jurkat whole cell lysates. Lane 2: A431 whole cell lysates. Lane 3: THP-1 whole cell lysates. Lane 4: 293T/17 whole cell lysates. Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 22 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-BID Antibody at 1:500-2000 dilution. Lane 1: HeLa whole cell lysate. Lane 2: A431 whole cell lysate. Lane 3: MDA-MB-231 whole cell lysate. Lane 4: Raji whole cell lysate. Lane 5: Jurkat whole cell lysate. Lane 6: THP-1 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 22 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining BID in human bladder sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (human lung adenocarcinoma epithelial cell line) cells labeling BID at 1/25 dilution, followed by Dylight 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on A549 cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).

Quick Database Links

Gene Symbol

BID

UniProt

P55957