Bovine FGF basic protein

SKU: orb80023

Description

Bovine FGF basic protein

Images & Validation

• Tested Applications: SDS-PAGE
• Application Notes:
  < strong>Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.85 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).2. Analysis by RP-HPLC, using calibrated solution of FGF-2 Bovine as Reference Standard.

  Read more...

Key Properties

• Biological Origin: E.coli
• Biological Activity: The ED50, measured in mitogenic assay using quiescent NR6R-3T3 fibroblasts was found to be < 0.1 ng/ml, corresponding to specific activity of 3,000,000 Units/mg.
• Target: Bovine FGF basic
• Conjugation: Unconjugated
• Purity: > 97.0% as determined by RP-HPLC and analysis by SDS-PAGE
• Protein Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Ala-Gly-Ser.

Storage & Handling

• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Buffer/Preservatives: The FGF-b Bovine was lyophilized from concentrated (1mg/ml) sterile solution containing 1%HSA.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HBGH-2 protein, HBGF-2 protein, Prostatropin protein

Quick Database Links

Gene Symbol

Bovine FGF basic