BRAF Antibody

SKU: orb2316280

Description

BRAF Antibody

Research Area

,B Cell Markers|Bladder Cancer|Breast Cancer|Cardiovascular|Colon Cancer|Infectious Disease|MAPK Signaling|Signal Transduction

Images & Validation

• Tested Applications: IHC
• Dilution Range: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Reactivity: Human
• Application Notes:
  Positive Control: A431, Jurkat and HeLa cells. Melanoma or Colon Carcinoma.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: BRAF/1626
• Immunogen: Recombinant fragment of human HMG20B protein (exact sequence is proprietary)
• Target: HMG20B
• Source/Expression System: Bioreactor
• Molecular Weight: 35kDa (predicted)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Concentration: Purified Ab WITHOUT BSA at 1.0mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BRAF25; BRAF35; BRCA2-associated factor 35; High Mobility Group Protein 20B (HM20B); HMG box-containing protein 20B; HMG domain-containing protein HMGX2; HMG20B; SMARCE1-related protein; SMARCE1r; Sox-like transcriptional factor; SOXL

Quick Database Links

Gene Symbol

HMG20B

UniProt

Q9P0W2

NCBI Gene ID

673