C-myc mRNA-LNP

SKU: orb2719962

Description

Myc is a family of regulatory genes and proto-oncogenes encoding transcription factors. The Myc family consists of three related human genes: c-myc (MYC), l-myc (MYCL) and n-myc (MYCN). c-myc (sometimes also called MYC) was the first gene identified in this family because of its homology to the viral gene v-myc. The transcription factor C-myc is a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis, and cellular transformation. C-myc regulates the transcription of specific target genes by forming heterodimers with the related transcription factor MAX and binding the E-box DNA consensus sequence. In cancer, c-myc is often constitutively expressed, leading to increased expression of many genes, some of which are involved in cell proliferation and contribute to cancer development. There is evidence that C-myc translation can be initiated from upstream out-of-frame AUG (CUG) and downstream AUG initiation sites, resulting in two isoforms with distinct N-termini. This product is designed as a tool for the delivery and expression of C-myc mRNA for research. The product leverages the lipid nanoparticle (LNP) technology platform for simple and efficient delivery of C-myc mRNA to a variety of mammalian cells in vitro and in vivo. The LNPs used are formulated with SM-102, DSPC, cholesterol and DMG-PEG2000 at an optimal molar concentration for a high rate of encapsulation and efficient mRNA delivery. The C-Myc protein is about 50 kD and consists of 454 amino acids. The GenPept accession number is NP_002458.

Images & Validation

• Application Notes:
  Upon receiving product, briefly pulse spin before opening to ensure product is at bottom of container. It is important not to spin for too long as this may rupture mRNA-LNPs. Do not vortex. Work with mRNA-LNPs on ice and minimize the time that the product spends at room temperature. After handling the product during experiments, return immediately to ice. mRNA-LNP products should only be handled with certified RNase-free reagents and consumables. Use of filtered pipette tips is highly recommended.

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Storage & Handling

• Storage: 4°C; ice
• Form/Appearance: mRNA-LNPs suspended in PBS (-Ca, -Mg) (pH: 7.0-7.4).
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only