CA125 Rabbit Polyclonal Antibody

SKU: orb414535

Description

CA125 Rabbit Polyclonal Antibody

Research Area

Product Categories/Products/Antibodies,MUC,Tumor biomarkers,Tumor antigens,MUC

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CA125 (12600-12650/14507aa)
• Target: MUC16
• Molecular Weight: 200-270 kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CA 125; CA-125; CA125 ovarian cancer antigen; Cancer antigen 125; FLJ14303; MUC16_HUMAN; MUC 16; MUC16; Mucin 16; MUC-16; Mucin 16 cell surface associated; Mucin16; Ovarian cancer related tumor marker CA125; Ovarian carcinoma antigen CA125; Ovarian cancer-related tumor marker CA125.

Paraformaldehyde-fixed, paraffin embedded (Human cervical cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CA125) Polyclonal Antibody, Unconjugated (orb414535) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human stomach cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CA125) Polyclonal Antibody, Unconjugated (orb414535) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Sample: Siha (Human) Cell Lysate at 40 ug, Primary: Anti-CA125 (orb414535) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 200 kD, Observed band size: 230 kD.

Quick Database Links

Gene Symbol

MUC16

UniProt

Q8WXI7