CALD1 (vPairTM) Antibodies

SKU: orb131724

Description

Rabbit polyclonal to CALD1. CALD1 antibodies represent a pair of fully characterized antibodies that recognize two different regions of a target protein. The product is developed by Biorbyt to address whether the signal observed truly represents the protein of interest, an often encountered issue in antibody pairs-based assays. The use of a pair of fully characterized vPair antibodies in the same assay can validate signal specificity since vPair antibodies recognize two independent epitopes of the same protein. Different sets of vPair antibodies are developed at Biorbyt to work with specific applications, including antibody pairs arrays, Western blot, IP-Western, ChIP, IHC, and FACS. Caldesmon (CALD1) belongs to the caldesmon family and is an actin-and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). CALD1 stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, CALD1 inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin.

Research Area

Product Categories/Products/Antibodies,Epigenetics,Product Categories/Products/Antibodies/Primary Antibodies

Images & Validation

• Tested Applications: WB
• Dilution Range: WB:1:1000-1:3000
• Reactivity: Human, Mouse
• Application Notes:
  < strong>WB: The apparent protein size on WB may be different from the calculated M.W. due to modifications.; < br>< strong>Experiment Notes: Rabbit polyclonal antibodies were produced by immunizing animals with GST-fusion proteins containing either the N-terminal or the C-terminal region of human CALD1.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: N-terminal or C-terminal region of human CALD1
• Molecular Weight: 93
• Purity: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: -20°C
• Buffer/Preservatives: 0.01% NaN3
• Concentration: 100 μg/ml BSA
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti Caldesmon antibody pairs, anti CDM antibody pairs, anti CAD antibody pairs

Quick Database Links

UniProt

Q05682