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CaM Kinase II (phospho Thr286) Antibody

SKU: orb1273535

Description

CaM Kinase II (phospho Thr286) Antibody

Research Area

,Signal Transduction,Phospho-Specific

Images & Validation

Tested ApplicationsWB
ReactivityFrog, Human, Mouse, Rat
Application Notes
Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state. Western blots of rat brain lysates with phospho- and dephosphopeptide competition were used to establish the phospho-specificity of the antibody. Applications include Dot Blot (DB) and Western Blot (WB). Dot Blots and Western Blots performed with each lot and working dilutions internally tested under ideal conditions for DB and WB were 1:1000. Suitability for Immunohistochemistry (IHC) has not been determined. Human, mouse, rat and Xenopus have 100% amino acid sequence identity with the antigen used to raise the antibody.

Key Properties

Antibody TypePrimary Antibody
ClonalityPolyclonal
ImmunogenCaM Kinase II (Thr286) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding the phosphorylated-Thr286 found in rat brain CaM Kinase II.
TargetCamk2a
Molecular Weight50/60
PurificationAffinity Purified
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Concentrationbatch dependent
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

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UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product
RefSeqP11275

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol

Available Sizes

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0.1 ml
$ 960.00
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 5-10 working days
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