CaMKII (Alpha-Specific) Antibody: RPE

SKU: orb147046

Description

Mouse monoclonal to CaMKII (RPE). CaMKII is an important member of calcium/calmodulin- activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling. CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca2+ auto inhibitory effect and activates the kinase. /calmodulin to its regulatory domain releases its This kinase activation results in auto phosphorylation at threonine 286. The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation. Auto phosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12)..

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Cardiovascular Disease,Neuroscience,Epigenetics,Cell Signaling | Post-translational Modifications | Phosphorylation

Images & Validation

• Tested Applications: ICC, IF, IHC, WB
• Dilution Range: WB (1:10000), IHC (1:2000), ICC/IF (1:50)
• Reactivity: Bovine, Human, Mouse, Rat
• Application Notes:
  0.1 µg/ml was sufficient for detection of CamKII in 20 µg rat brain tissue extract by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 6G9
• Immunogen: Partially purified rat CaMKII
• Target: CaMKII
• Molecular Weight: 50-60kDa
• Conjugation: RPE

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 95.64mM Phosphate, 2.48mM MES and 2mM EDTA
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CamK2 antibody, CamK2A antibody, CamK2B antibody, CamK2D antibody, CamK2G antibody, CAMKA antibody

Quick Database Links

Gene Symbol

CaMKII

UniProt

P11798

NCBI Gene ID

12322

RefSeq (Protein):NP_033922.1