Canine TNF-alpha Protein, His Tag

SKU: orb762406

Description

Canine TNF-alpha, His Tag is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # P51742-1).

Research Area

Immunology & Inflammation

Images & Validation

• Application Notes:
  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 18.6 kDa. The protein migrates as 19-22 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

• Expression System: HEK293
• Biological Origin: Canine
• Biological Activity: Immobilized Humira at 5 µg/mL (100 µL/well) can bind Canine TNF-alpha, His Tag with a linear range of 0.3-1 ng/mL (QC tested).
• Target: TNF-alpha
• Reactivity: Canine
• Tag: C-6×His
• Expression Region: Val 77 - Leu 233
• Endotoxins: 1.0 EU per μg
• Conjugation: Unconjugated
• MW: 18.6 kDa
• Purity: 90%
• Protein Sequence: NP_001003244.4

Storage & Handling

• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Immobilized Humira at 5 μg/mL (100 μL/well) can bind Canine TNF-alpha, His Tag (Cat. No. orb762406) with a linear range of 0.3-1 ng/mL (QC tested).

Quick Database Links

Gene Symbol

TNF-alpha

UniProt

P51742

RefSeq (Protein):NP_001003244.4