CASP8 Antibody (C-term)

SKU: orb349639

Description

Mouse monoclonal antibody to Caspase 8.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Cancer,Neuroscience,Signaling Pathways,Product Categories/Products/Antibodies/Primary Antibodies,Signaling Pathways/Apoptotic,Epigenetics,Infectious Diseases,Crown Antibodies;Primary Antibodies;Cancer;Cell Biology;Metabolism;Host-virus interaction;Inflammasome;RIG-l-like Receptor;Toll-Like receptor signaling;Immune system process;Apoptosis;Apoptosis signaling;Necroptosis

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: WB: 1:2000
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 550CT8.5.2
• Immunogen: Synthesized Peptide
• Target: CASP8
• Molecular Weight: 55391
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti Apoptosis related cysteine peptidase antibody, anti Apoptotic cysteine protease antibody, anti Apoptotic protease Mch 5 antibody, anti Apoptotic protease Mch-5 antibody, anti Apoptotic protease Mch5 antibody, anti CAP 4 antibody, anti CAP4 antibody, anti CASP 8 antibody, anti CASP-8 antibody, anti CASP8 antibody, anti Caspase 8 apoptosis related cysteine peptidase antibody, anti Caspase-8 subunit p10 antibody, anti Caspase8 antibody, anti CED 3 antibody, anti FADD homologous ICE/CED 3 like protease antibody, anti FADD Homologous ICE/CED3 Like Protease antibody, anti FADD Like ICE antibody, anti FADD-homologous ICE/CED-3-like protease antibody, anti FADD-like ICE antibody, anti FLICE antibody, anti ICE like apoptotic protease 5 antibody, anti ICE-like apoptotic protease 5 antibody, anti MACH antibody, anti MORT1 associated CED 3 homolog antibody, anti MORT1 associated CED3 homolog antibody, anti MORT1-associated CED-3 homolog antibody

All lanes: Anti-CASP8 Antibody (C-term) at 1:2000 dilution. Lane 1: HepG2 whole cell lysate. Lane 2: Jurkat whole cell lysate. Lane 3: HL-60 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 55 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Overlay histogram showing Jurkat cells stained (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was mouse IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

Quick Database Links

Gene Symbol

CASP8

UniProt

Q14790