Caspase-3 cleaved p17/19

SKU: orb1294355

Description

Rabbit polyclonal antibody to Caspase 3 p17

Research Area

,Product Categories/Antibodies,Apoptosis / Caspase

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: Western Blot 1:1000 Immunofluorescence 1:400 – 1:500 Immunohistochemistry (Paraffin) 1:100 – 1:300
• Reactivity: Human, Mouse, Rabbit

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the C-terminus region.
• Target: Caspase-3 cleaved p17/19
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

This blot was produced using a 12% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294355 overnight at 4°C. All lanes: Anti-Caspase 3 antibody - at 1/800 dilution, Lysates/proteins at 50 μg per lane.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294355 Anti-Caspase 3 p17/19 antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Caspase 3 p17/19 with orb1294355 at 1/200.

This blot was produced using a 12% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294355 overnight at 4°C. All lanes: Anti-Caspase 3 antibody - at 1/800 dilution, Lysates/proteins at 50 μg per lane.

This blot was produced using a 15% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294355 overnight at 4°C. Anti-Caspase 3 antibody - at 1/1000 dilution, Lysates at 30 μg per lane.

Quick Database Links

Gene Symbol

Caspase-3 cleaved p17/19